Antihelminthic action of the Anethum graveolens essential oil on Haemonchus contortus eggs and larvae

Abstract Gastrointestinal nematodes are responsible for great economic losses in sheep raising, and their control has long been carried out almost exclusively by the administration of anthelmintics, which have led to serious resistance problems. In search alternative measures, phytotherapic research is highlighted. The aim this study was evaluate action Anethum graveolens (dill) essential oil on different stages Haemonchus contortus life cycle, as well its cytotoxicity MDBK (Madin-Darby bovine kidney) cells. H. larvae eggs were obtained from infected feces, extracted plant seeds through Clevenger apparatus. 9.4, 4.7, 2.35, 1.17. 0.58 0.29 mg/mL concentrations evaluated. Egg Hatch Inhibition (HI), Larval Development (LDI) Migration (LMI) techniques used. Thybendazole 0.025 HI Levamisole 0.02 LDI LMI tests used positive controls, while distilled water a Tween 80 solution negative controls. inhibition results highest concentration were: 100%, 98.58% 63.7%, differing (�� <0.05) Main A. components present 95.93% total Dihydrocarvone (39.1%), Carvone (22.24%), D-Limonene (16.84%), Apiol (10.49%) Trans-dihydrocarvone (7.26%). Minimum required inhibit 50% (IC50) egg hatching, larval development migration 0.006 mg/mL, 2.536 3.963 respectively. Cell viability cells, when incubated with oil, 86% (9.4 mg/mL) 99% lowest (0.29 mg/mL). according study, promising gastrointestinal nematode control.